The hydrolysis of tributyrin was tracked by TLC and a strain named T6 which can produce lipase with non special catalysis was obtained. It is identified as Proteus vulgaris by the identification method of configuration, physiology, biochemistry, molecular biology. 利用薄层层析法跟踪脂肪酶水解甘油三酯过程,根据产物判断脂肪酶催化位置特异性,得到一株催化位置非特异性脂肪酶产生菌T6,经形态,生理生化和分子生物学鉴定该菌株为Proteusvulgaris。